Pubmed link to all publications


*undergraduate co-authors

57) Pokhrel N, Origanti S, Davenport EP, Gandhi D, Kaniecki K, Mehl RA, Greene EC, Dockendorff C, Antony E. 2017. Monitoring replication protein a (RPA) dynamics in homogous recombination through site-specific incorporation of non-canonical amino acids. Nucleic Acids Research, in press Doi 10.1093/narlgkx598.

56) Blizzard, R.J., Gibson, T.E., and Mehl, R.A. 2017. Site Specific Protein Labeling with Tetrazine Amino Acids. Methods Mol. Biol. In press

55) Alghamdi M, Zhang J. Oswalt A. Porter JJ, Mehl RA, Kong W. 2017. Doping of green fluorescent protein into superfluid helium droplets: Size and velocity of Doped Droplets, J. of Phy.Chem. A. July 12 accepted.

54) Row RD, Shih HW, Alexander AT, Mehl RA, Prescher JA. 2017. Cyclopropenones for metabolic targeting and sequential bioorthogonal labeling. J Am Chem Soc.  May 31;139(21):7370-7375.

53) Sungwienwong I, Hostetler ZM, Blizzard RJ, Porter JJ, Driggers CM, Mbengi LZ, Villegas JA, Speight LC, Saven JG, Perona JJ, Kohli RM, Mehl RA, Petersson EJ. 2017. Improving target amino acid selectivity in a permissive amino acyl tRNA synthetase through counter-selection. Org Biomol Chem. May 3;15(17):3603-3610.

52) Scholfield MR, Ford MC, Carlsson AC, Butta H, Mehl RA, Ho PS.  2017. Structure-Endergy Relationship of Halogen Bonds in Proteins. Biochemistry. 56(22):2794-2802.

51) Averick, S, Matyjaszewski, K., Mehl, R. 2016, Genetically Encoded Initiator For Polymer Growth From Proteins. PCT US 9,243,274.

50) Gu X, Wu Z, Huang Y, Wagner MA, Baleanu-Gogonea C, Mehl RA, Buffa JA, DiDonato AJ, Hazen LB, Fox PL, Gogonea V, Parks JS, DiDonato JA, Hazen SL. A systematic investigation of structure/function requirements for the apolipoprotein A-I - lecithin cholesterol acyltransferase interaction loop of HDL. J Biol Chem. 2016 Jan 21. pii: jbc.M115.696088.

49) Rauch BJ, Porter JJ, Mehl RA, Perona JJ. Improved Incorporation of Noncanonical Amino Acids by an Engineered tRNATyr Suppressor. Biochemistry, 2016; 55(3); 618-28.

48) Leisle L, Valiyaveetil F, Mehl RA, Ahern CA. Incorporation of Non-Canonical Amino Acids. Adv Exp Med Biol. 2015; 869:119-51.

47) Blizzard RJ, Backus DR*, Brown W*, Bazewicz CG.*, Li Y, Mehl RA. Ideal Bioorthogonal Reactions Using A Site-Specifically Encoded Tetrazine Amino Acid. J Am Chem Soc. 2015; 137:10044-7.

46) Franco MC, Ricart KC, Gonzalez AS, Dennys CN, Nelson PA, Janes MS, Mehl RA, Landar A,  and Estevez AG. (2015) Nitration of Hsp90 on tyrosine 33 regulates mitochondrial metabolism. J Biol Chem. 290(31):19055-66.

45) Kamber DN, Liang Y, Blizzard RJ, Liu F, Mehl RA, Houk KN, and Prescher JA. (2015) 1,2,4-Triazines Are Versatile Bioorthogonal Reagents. J Am Chem Soc. 137(26):8388-91.

44) Averick S, Mehl RA, Das SR, and Matyjaszewski K. (2015) Well-defined biohybrids using reversible-deactivation radical polymerization procedures, J Control Release. 10;205:45-57.

43) Darko, A., Wallace, S., Dmitrenko, O., Machovina, M. M.*, Mehl, R. A., Chin, J. W., and Fox, J. M. (2014) Conformationally strained trans-cyclooctene with improved stability and excellent reactivity in tetrazine ligation, Chem Sci 5, 3770-3776.

42) Averick, S.E., Bazewicz, C.G.*, Woodman, B.F.*, Simakova, A., Mehl, R.A., Matyjaszewski, K. (2014) Protein-Polymer Hybrids: Conducting ARGET ATRP from a Genetically Encoded Cleavable ATRP Initiator, European Polymer Journal 49, (10) 2919–2924

41) Chatterjee, D., Cooley, R. B., Boyd, C. D., Mehl, R. A., O'Toole, G. A., and Sondermann, H. (2014) Mechanistic insight into the conserved allosteric regulation of periplasmic proteolysis by the signaling molecule cyclic-di-GMP, eLife 3, e03650.

40) Cooley, R.B., Karplus, P.A., & Mehl, R.A. (2014) Gleaning Unexpected Fruits from Hard-Won Synthetases: Probing Principles of Permissivity in Non-canonical Amino Acid-tRNA Synthetases. Chembiochem, 15(12), 1810-9.

39) Averick, S., Karácsony, O., Mohin, J., Yong, X., Moellers, N.M., Woodman, B.F., Zhu, W., Mehl, R.A., Balazs, A.C., Kowalewski, T., & Matyjaszewski, K. (2014). Cooperative, reversible self-assembly of covalently pre-linked proteins into giant fibrous structures. Angew Chem Int Ed Engl, 53(31), 8050-5.

38) Padmanarayana, M., Hams, N., Speight, L.C., Petersson, E.J., Mehl, R.A., & Johnson, C.P. (2014). Characterization of the Lipid Binding Properties of Otoferlin Reveals Specific Interactions between PI(4,5)P2 and the C2C and C2F Domains. Biochemistry, 53(30), 5023-33

37) Horowitz, S., Adhikari, U., Dirk, L.M., Del Rizzo, P.A., Mehl, R.A., Houtz, R.L., Al-Hashimi, H.M., Scheiner, S., & Trievel, R.C. (2014). Manipulating Unconventional CH-Based Hydrogen Bonding in a Methyltransferase via Noncanonical Amino Acid Mutagenesis. ACS Chem Biol., 9(8), 1692-1697

36) Wang, K., Sachdeva, A., Cox, D., Wilf, N., Wallace, S., Mehl, R., & Chin, J. (2014). Optimized orthogonal translation facilitates genetically directed, rapid and spontaneous protein double labeling for FRET. Nature Chemistry, 6(5), 393-403.

35) Cooley, R., Feldman, J.*, Driggers, C., Bundy, T.*, Stokes, A.*, Karplus, P.A., & Mehl, R. (2014). Structural basis of improved second generation 3-nitro-tyrosine tRNA synthetases. Biochemistry, 53(12), 1916-1924.

34) DiDonato, J., Aulak, K., Huang, Y., Wagner, M., Gerstenecker, G., Gogonea, V., Tang, W.H.W., Mehl, R., Plow, E.F., Smith, J., Fisher, E., & Hazen, S. (2014). Site-specific nitration of apolipoproteinA1 at tyrosine 166 is abundant within human atherosclerotic plaque and is dysfunctional. J. Biol. Chem, 289(15), 10276-10292.

33) Speight, L., Muthusamy, A., Goldberg, J., Warner, J.B., Wissner, R., Willi, T., Woodman, B.*,  Mehl, R., & Petersson E.J. (2013). Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Forster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies. J. Am. Chem Soc., 135(50), 18806-18814.

32) Horowitz, S., Dirk, L.M., Yesselman, J.D., Nimtz, J.S., Adhikari, U., Mehl, R.A., Scheiner, S., Houtz, R.L., Al-Hashimi, H.M., Trievel, R.C. (2013). Conservation and Functional Importance of Carbon-Oxygen Hydrogen Bonding in AdoMet-Dependent Methyltransferases. J. Am. Chem Soc., 135(41):15536-48.

31) Wu, I.L., Patterson, M.A., Carpenter Desai, H.E., Mehl, R.A., Giorgi, G., Conticello, V.P. (2013). Multiple site-selective insertions of noncanonical amino acids into sequence-repetitive polypeptides. Chembiochem, 14(8), 968-78.

30) Maria Clara Franco, Yaozu Ye, Christian A. Refakis*, Jessica L. Feldman*, Audrey L. Stokes*, Manuela Basso, Raquel M. Melero Fernández de Mera, Nicklaus A. Sparrow, Noel Y. Calingasan, Mahmoud Kiaei, Timothy W. Rhoads, Thong Ma, Martin Grumet, Stephen Barnes, M. Flint Beal, Joseph S. Beckman, Ryan Mehl and Alvaro G. Estévez. Nitration of Hsp90 induces cell death. PNAS 2013, 110(12) E1102-E1111.

29) Jason L. Seitchik*, Jennifer C. Peeler*, Michael T. Taylor, Melissa L. Blackman, Timothy W. Rhoads, Richard B. Cooley, Christian Refakis*, Joseph M. Fox, and Ryan A. Mehl. Genetically Encoded Tetrazine Amino Acid Directs Rapid Site-Specific in Vivo Bioorthogonal Ligation with trans-Cyclooctenes. J. Am. Chem. Soc. 2012, 134(6), 2898-901.

28) Ryan A. Mehl. Engineered Unnatural Animals: Tools for Multicellular Biochemistry. ChemBioChem. 2012 13(2), 186-8.

27) Saadyah Averick, Antonina Simakova, Sangwoo Park, Dominik Konkolewicz, Andrew J. D. Magenau, Ryan A. Mehl, and Krzysztof Matyjaszewski. ATRP under Biologically Relevant Conditions: Grafting from a Protein. ACS Macro Letters, 2012, 1, 6-10.

26) Saadyah Averick, Eduardo Paredes, Debasish Grahacharya,  Bradley F. Woodman*, Shigeki J. Miyake-Stoner*, Ryan A. Mehl, Krzysztof Matyjaszewski, Subha R. Das. A Protein-Polymer Hybrid Mediated By DNA. Langmuir 2012, 28(4), 1954-8.

25) R. Daniel Libby,  Ryan A. Mehl. Characterization of covalent Ene adduct intermediates in ‘‘hydride
equivalent’’ transfers in a dihydropyridine model for NADH reduction reactions. Bioorganic Chemistry. 2012, 40(1), 57-66.

24) Saadyah Averick, Antonina Simakova, Andrew Magenau, Andrew Seong, Bradley F. Woodman*, Ryan A. Mehl, Krzysztof Matyjaszewski.  Covalently Incorporated Protein-Nanogels using AGET ATRP in Inverse Miniemulsion. Polymer Chem. Comm. 2011, 2 (7), 1476-1478.

23) Jennifer C. Peeler*, Ryan A. Mehl. Methods in Molecular Biology series, “Unnatural amino acids”. Site-Specific incorporation of unnatural amino acids as probes for protein conformational changes Springer Science, Methods in Molecular Biology 2012. 794, 125-134.

22) Jennifer C. Peeler*, Bradley F. Woodman*, Saadyah Averick, Shigeki J. Miyake-Stoner*, Audrey L. Stokes*, Krzysztof Matyjaszewski, Ryan A. Mehl. Genetically Encoded Initiator For Polymer Growth From Proteins. J. Am. Chem. Soc. 2010, 132(39), 13575.

21) Laura Rowe, Mark Ensor, Ryan A. Mehl and Sylvia Daunert. Modulating the Bioluminescence Emission of Photoproteins by in Vivo Site-Directed Incorporation of Non-Natural Amino Acids. ACS Chem. Biol., 2010, 5(5), 455-460.

20) Shigeki J. Miyake-Stoner*, Christian A. Refakis*, Jared T. Hammill*, Hrvoje Lusic, Alexander Deiters, Jennifer L. Hazen*, Ryan A. Mehl. 2010. Generating Permissive Site-specific Unnatural Aminoacyl-tRNA Synthetases. Biochemistry, 2010, 49 (8), 1667-1677.

19) Conggang Li,† Gui-Fang Wang,† Yaqiang Wang,† Rachel Creager-Allen, Evan A. Lutz, Heidi Scronce, Kristin M. Slade, Rebecca A.S. Ruf, Ryan A. Mehl, Gary J. Pielak. Protein 19F NMR in Escherichia coli. J. Am. Chem. Soc. 2010, 132, 321–327.

18) Taskent-Sezgin, Humeyra; Chung, Juah; Patsalo, Vadim; Miyake-Stoner, Shigeki J.*; Miller, Andrew M.*; Brewer, Scott H.; Mehl, Ryan A.; Green, David F.; Raleigh, Daniel P.; Carrico, Isaac. 2009. Interpretation of p-Cyanophenylalanine Fluorescence in Proteins in Terms of Solvent Exposure and Contribution of Side-Chain Quenchers: A Combined Fluorescence, IR and Molecular Dynamics Study. Biochemistry, 48(38), 9040-9046.

17) Miyake-Stoner, S.*; Miller, Andrew A.; Hammill, J. T*.; Peeler, Jennifer C*.; Hess K. R.; Mehl, Ryan A.; Brewer, Scott H. 2009. Probing Protein Folding Using Site-Specifically Encoded Unnatural Amino Acids as FRET Donors with Trp. Biochemistry 48, 5953-5962.

16) Stokes, Audrey L.*; Miyake-Stoner, Shigeki J.*; Peeler, Jennifer C.*; Nguyen, Duy P.*; Hammer, Robert P.; Mehl, Ryan A.  2009.  Enhancing the Utility of Unnatural Amino Acid Synthetases by Manipulating Broad Substrate Specificity.  Mol. BioSyst.  5, 1032-1038.

15) Neumann, Heinz; Hazen, Jennifer L*.; Weinstein, John*; Mehl, Ryan A.; Chin, Jason W. 2008. Genetically Encoding Protein Oxidative Damage. J. Am. Chem. Soc. 130(12),  4028-4033.

14) Carrico, Zachary M.; Romanini, Dante W.; Mehl, Ryan A.; Francis, Matthew B. 2008.   Oxidative coupling of peptides to a virus capsid containing unnatural amino acids. Chemical Communications (10), 1205-1207.

13) Hammill, J. T*.; Miyake-Stoner, S.*; Hazen, J. L.*; Jackson, J. C.*; Mehl, R. A. 2007. Preparation of site-specifically labelled fluorinated proteins for 19F-NMR structural characterization. Nat. Protoc. 2(10), 2601-2607.

12) Mehl, R. 2007. Site-specific incorporation of fluorinated amino acids into proteins PCT US, 268 pp.

11) Jackson, J. C*.; Hammill, J. T.*; Mehl, R. A. 2007. Site-Specific Incorporation of a 19F-Amino Acid into Proteins as an NMR Probe for Characterizing Protein Structure and Reactivity. J. Am. Chem. Soc. 129(5), 1160-1166.

10) Jackson J. C.*; Duffy S. P.*; Hess K. R.; Mehl R. A. 2006. Improving Nature’s Active Site with Genetically Encoded Unnatural Amino Acids. J. Am. Chem. Soc. 128(34), 11124-11127.

9) Farrell I. S.*; Toroney R.*; Hazen J. L.*; Mehl R. A.; Chin J. W. 2005. Photo-cross-linking interacting proteins with a genetically encoded benzophenone. Nature Methods. Vol. 2, No. 5, 377-384.

8) Lawhorn, B. G.; Mehl, R. A.; Begley, T. P. 2004. Biosynthesis of the thiamin pyrimidine: The reconstitution of a remarkable rearrangement reaction. Organic & Biomolecular Chemistry. Vol. 2, 1-9.

7) Mehl, R. A.; Anderson, J. C.; Santoro, S.; Wang, L.; Martin, A.; King, D. S.; Horn D. M.; Schultz, P. G. 2003. Generation of a Twenty-one Amino Acid Bacterium. J. Am. Chem. Soc. 125(4), 935-939.

6) Schultz, P.; Wang, L.; Anderson, J. C.; Chin, J. W.; Liu, D. R.; Magliery, T. J.; Meggers, E. L.; Mehl, R. A.; Pastrnak, M.; Santoro, S. W.; Zhang, Z. 2002. Engineering of mutant orthogonal tRNA - aminoacyl tRNA synthetase pairs from Methanococcus jannaschii for incorporation of unnatural amino acids into proteins in vivo. PCT Int. 170 pp.

5) Schultz, P.; Wang, L.; Anderson, J. C.; Chin, J. W.; Liu, D. R.; Magliery, T. J.; Meggers, E. L.; Mehl, R. A.; Pastrnak, M.; Santoro, S. W.; Zhang, Z. 2002. Use of non-native tRNAs and amino acyl tRNA synthetases with relaxed substrate specificity in the in vivo incorporation of unnatural amino acids. PCT Int. 188 pp.   

4) Mehl, R. A.; Begley, T. P. 2002. Synthesis of 32P-labeled intermediates on the purine biosynthetic pathway. J. Labelled Compounds and Radiopharmaceuticals. 45(13), 1097-1102.

3) Begley, T. P.; Kinsland, C.; Mehl, R. A.; Osterman, A.; Dorrestein, P. 2001. The biosynthesis of nicotinamide adenine dinucleotides in bacteria. Vitamins and Hormones (Academic Press, San Diego, CA, United States), Vol. 61, pp. 103-119. ISSN:0083-6729.

2) Mehl, R. A.; Kinsland, C.; Begley, T. P. 2000. Identification of the Escherichia coli nicotinic acid monomucleotide adenylytransferase gene. J. Bacteriology. 182(15), 4372-4374.

1) Mehl, R. A.; Begley, T. P. 1999. Mechanistic studies on the repair of the novel DNA photolesion: The spore photoproduct. Organic Letters. 1(7), 1065-1066.